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991.
992.
采用3×3因子方法,进行了饲料中不同蛋白质和脂肪水平对克氏螯虾f均重(7.03±0.05)g]生长性能、体组成和消化酶活性的影响研究。试验设3个蛋白质水平(24%、27%和30%1和3个脂肪水平(4%、7%和10%),共9组,每组4个重复,每个重复10尾虾,为期8周。结果表明,蛋白质和脂肪水平对成活率无显著影响(P〉0... 相似文献
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The spatial distribution of ammonia-oxidizing Betaproteobacteria ( β AOB) was investigated by FISH (fluorescence in situ hybridization) and DGGE (denaturing gradient gel electrophoresis) techniques in the sediment off the Changjiang River Estuary.Sediment samples were collected from eight stations in June before the formation of hypoxia zone in 2006.The abundance of β AOB ranged from 1.87 × 10 5 to 3.53 × 10 5 cells/g of sediment.β AOB abundance did not present a negative correlation with salinity,whereas salinity was implicated as the primary factor affecting nitrification rates.The DGGE profiles of PCR-amplified amo A gene fragments revealed that the β AOB community structure of sample S2 separated from other samples at the level of 40% similarity.The variations in composition of β AOB were significantly correlated with the salinity,temperature,absorption ability of sediments and TOC. The statistical analysis indicates that the β AOB abundance was a main factor to influence nitrification rates with an influence ratio of 87.7% at the level of 40% biodiversity similarity.Considering the good correlation between β AOB abundance and nitrification estimates,the abundance and diversity of β AOB community could be expected as an indirect index of nitrification activity at the study sea area in summer. 相似文献
996.
Ten highly variable microsatellite loci were performed in order to evaluate the genetic variability of wild and hatchery samples of half-smooth tongue sole Cynoglossus semilaevis. A group of 200 genotypes belonging to four wild samples, Laizhou (LZ), Weihai (WH), Qingdao (QD), Rizhao (RZ) and one hatchery sample, Mingbo (MB), were screened. All of the ten microsatellite loci screened in this study showed marked polymorphism. A total of 70 different alleles were observed over all loci. The number of alleles per locus ranged from 4.2 to 10.6. The average of expected and observed heterozygosity ranged from 0.67 to 0.82, and from 0.77 to 0.87, respectively. A total of 10, 16, 10, 11 and 5 unique alleles each were found in LZ, WH, QD, RZ and MB populations. The effective number of alleles varied from 2.87 for HSTS_7 to 6.83 for HSTS-h. The number of average genotypes ranged from 6.0 for HSTS_a to 15.4 for HSTS_h. As compared with the wild populations, the hatchery population, MB, showed significant genetic changes such as fewer alleles per locus (P <0.05), a smaller number of low frequency alleles (P <0.05), a small number of unique alleles and a small number of genotypes (P <0.05), all indicative of a reduction in genetic diversity. 相似文献
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Low-frequency variability of the North Equatorial Current bifurcation in the past 40 years from SODA 总被引:1,自引:1,他引:0
The low-frequency variability of the North Equatorial Current (NEC) bifurcation during 1958 to 2001 was investigated with the Simple Ocean Data Assimilation (SODA) 2.0.2 dataset.In agreement with recent observations,the NEC bifurcation latitude (NBL) shifted northward as depth increases, from about 12.7°N near the surface to about 17.1°N at depths around 500 m for the annual average. This study reveals that the interannual variations of NBL,with five years period,mainly focused on the upper 500 m with amplitude increasing as depth increased.The NBL shifted southward in the past 40 years,which was more significant in the subsurface at more than -0.02°/a.The NBL manifests itself in the transports of NMK (NEC-Mindanao Current (MC)-Kuroshio) system in strong relationship with MC (0.7) and Kuroshio (-0.7).The EOF analysis of meridional velocity off the Philippine coast shows that the first mode,explaining 62% of variance and 5 years period,was highly correlated with the southward shift of NBL with coefficient at about 0.75.The southward shift of NBL consists with the weakening of MC and strengthening of Kuroshio,which exhibited linear trends at -0.24Sv/a and 0.11Sv/a.Both interannual variation and trend of NBL were closely related to the variation of NMK system. 相似文献
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